K2 killer toxin-induced physiological changes in the yeastSaccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Nystatin-induced changes in Saccharomyces cerevisiae.
Nystatin (5 to 10 mug/ml) was fungicidal to Saccharomyces cerevisiae NCYC 361. There was an initial rapid uptake of nystatin by the yeast cells at 30 C, the uptake being pH dependent, with a maximum at low pH values (3 to 4). Photomicrographs indicated that nystatin-treated cells became granular. The effect of growth temperature on the subsequent sensitivity of the yeast cells to nystatin was i...
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The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy, but it was unable to efflux 12K from the cells under these conditions. In dir...
متن کاملBinding of yeast killer toxin to a cell wall receptor on sensitive Saccharomyces cerevisiae.
35S-labeled killer toxin protein bound to cells of sensitive Saccharomyces cerevisiae S14a. Strains that were resistant to toxin through mutation in the nuclear genes kre1 kre2 bound toxin only weakly. Non-radioactive toxin competed effectively with 35S-labeled toxin for binding to S14a, but did not compete significantly in the binding to mutant kre1-1. This implied that binding to kre1-1 was ...
متن کاملProduction and Effect of Killer Toxin by Saccharomyces Cerevisiae on Sensitive Yeast and Fungal Pathogens
Killer toxins are the proteinaceous toxins produced by some group of yeast designatedKiller yeasts. These toxins have activity against microorganisms other than yeast and the activity is readily detectable only when a suitable sensitive strain is tested. The aim of this study was extract the Killer toxin from yeast cells, to purify by thin layer chromatography and to find out the action of kill...
متن کاملA Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin.
Using the set of Saccharomyces cerevisiae mutants individually deleted for 5718 yeast genes, we screened for altered sensitivity to the antifungal protein, K1 killer toxin, that binds to a cell wall beta-glucan receptor and subsequently forms lethal pores in the plasma membrane. Mutations in 268 genes, including 42 in genes of unknown function, had a phenotype, often mild, with 186 showing resi...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2016
ISSN: 1567-1364,1567-1364
DOI: 10.1093/femsyr/fow003